Parallel Plate Flow Chamber Cell Culture

Cells under flow the flow assay.

Parallel plate flow chamber cell culture.

In a parallel plate rheometer for a fluid such as oils or polymers the material is confined between the two shearing plates by capillary forces and does not flow out. Dishes are ready for use in flow assay. Prior to beginning flow assay. Refer to protocol 7.

On a more abstract level the device presents a platform that takes care of the well being of cells and the cassette like flow chamber creates an experimental environment that can be adjusted to individual needs. Fill system with media and remove all air from system. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and or reagents. Usually the torque is measured on the rotating disc.

3 i used different microfluidic device designs with higher aspect ratio to make them more similar to the parallel plate flow chambers including the ones that have been published by others and. The most common types of microchannels used in cell adhesion and flow studies are the radial flow chamber and the parallel plate flow chamber. Planning setup and analysis experimental workflow before starting choosing the optimal setup cells under flow. Assemble flow system apparatus connecting inlet outlet and vacuum lines to the flow chamber deck.

Although the here presented flow chamber follows a parallel plate design other chamber designs could be used as well. Parallel plate flow chamber experiments with endothelial cells have shown that shear flow can align cells 50 143 144 145 146 147 148. The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Flow assay using parallel plate flow chamber.

Turn on stage incubator and warm adhesion media to 37 c 1 hr. 3 2 1 parallel plate rheometer. Parallel plate flow chambers are used in dynamic studies of cell adhesion under well defined shear forces. Endothelial cells under flow for simulation of blood vessels ideal simulation of various physiological conditions continuous unidirectional oscillating and pulsatile flow.

A pump system for the cultivation of e g. Minimal mechanical stress minimal amount of medium and supplement needed. Several design modifications have occurred over the years yet a comparison of these different designs has not been performed. To understand the influence of fluid shear and to.

A parallel plate rheometer consists of two parallel discs one of which rotates. In addition the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. There are radial membrane minichannels for hematopoietic blood cell culture the bioartificial liver membranes enhance mass transfer in planar microchannels and oxygen and lactate transport in micro.